Journal: International Journal of Molecular Sciences

Article Title: Senescence Induces Dysfunctions in Endothelial Progenitor Cells and Osteoblasts by Interfering Translational Machinery and Bioenergetic Homeostasis

doi: 10.3390/ijms19071997

Figure Lengend Snippet: Effect of senescence on the content of ROS, superoxide, and antioxidant enzymes in OBCM-treated EPCs. ( A ) Young and senescent EPCs were incubated with OBCM of MC3T3-E1 cells for 24 h. Then, the production of ROS and superoxide was examined by ROS/superoxide detection kit ( N = 5); ( B , C ) Young and senescent EPCs were treated with or without OBCM of MC3T3-E1 cells for 24 h. After treatment, cells were harvested and lysed for the detection of antioxidant enzymes by Western blot analysis ( N = 5). The quantitative densitometry of the relative level of signals was performed with Image-Pro Plus. Data are expressed as mean ± S.E.M. of five independent experiments. * p < 0.05 compared with the basal group (without OBCM). # p < 0.05 compared with the group of young EPCs.

Article Snippet: After 24 h of incubation, the culture medium was removed and treated with OBCM for 24 h. ROS and superoxide production were then determined in live cells using ROS/superoxide detection kit (Enzo Life Sciences, Plymouth Meeting, Montgomery, PA, USA) according to the manufacturer’s instruction.

Techniques: Incubation, Western Blot

Journal: Journal of Advanced Research

Article Title: SAP deficiency aggravates periodontitis possibly via C5a-C5aR signaling-mediated defective macrophage phagocytosis of Porphyromonas gingivalis

doi: 10.1016/j.jare.2022.10.003

Figure Lengend Snippet: SAP deficiency aggravates periodontitis and inflammation. (a) Micro-CT 3D reconstruction images of the maxilla of wildtype and SAP-KO mice. (b) CEJ-ABC distance was analyzed from Micro-CT images (n = 5). (c) Histological images of H&E and TRAP staining, and representative CD34 and CD45 immunohistochemistry images of the periodontium in wildtype and SAP-KO periodontitis mice. Quantitative analysis of CEJ-ABC distance (d) and osteoclasts (e) from histological images (n = 5). Quantitative analysis of CD34 (f) and CD45 (g) positive cells from immunohistochemistry images (n = 3). (h-j) Inflammatory cytokine mRNA expression in the periodontium of wildtype and SAP-KO periodontitis mice (n = 3). (k-m) Protein level expression of inflammatory cytokines detected by ELISA (n = 5). Red dot line, CEJ level; Black dot line, ABC level; Blue double arrow line, CEJ-ABC distance. Yellow “R”, tooth root; Yellow “B”, alveolar bone. Black arrow, osteoclasts. Data are presented as mean ± SD. The significant difference among the groups, * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The protein level of SAP in the human gingival lysate was analyzed using the human SAP ELISA kit (Cusabio, Wuhan, China) and in mouse periodontal tissue lysate, liver lysate, and serum was analyzed using the mouse SAP ELISA kit (SEB539Mu, Cloud-Clone Corp, Wuhan, China).

Techniques: Micro-CT, Staining, Immunohistochemistry, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: In vitro assessment of the CD70-directed CAR-T cell anti-GB cytotoxicity (A) Measurement of secreted TNF-α and IFN-γ in the SN of GB/CAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. For comparison between MCS and CD70 (upper bar plots), an unpaired two-tailed t test was used. For comparisons among constructs (bottom), a one-way ANOVA followed by a Holm-Šídák multiple comparisons test was used. (B) Measurement of tumor cell signal during co-culture with CAR-T cells on the Incucyte platform. N = 2 biological replicates per group. Every biological replicate is the mean of N = 5 technical replicates. Time point intervals = 45 min. A two-tailed Student’s t test was performed using the values of the last measured time point to determine statistical significance. For (A) and (B), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Article Snippet: The following kits were used to quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA kit (#DY210, R&D Systems), and Mouse TNF-alpha DuoSet ELISA kit (#DY410, R&D Systems).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Comparison, Two Tailed Test, Construct, Co-Culture Assay

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: Evaluation of CD70-directed CAR-T cell effector function in cerebral organoids (A) Confocal microscopy of cerebral organoids, infiltrated by generated GB models. (B) Quantification of CD70 signal in organoids from (A). Each dot represents an organoid. A Welch’s t test was used to assess significance. (C) Immunofluorescence analysis of endogenous CD70 expression in cerebral organoids. (D) Confocal microscopy of cerebral organoids previously invaded by GB cells and subsequently treated with CAR-T cells for 3 d. (E) Quantification of Granzyme-B signal from (D). A two-tailed t test was used to determine significance. (F) Measurement of secreted Granzyme-B and IFN-γ levels in the SN of co-cultures from (D) by ELISA. N = 3 biological replicates per group. (G) CAR construct direct comparisons from (F). A one-way ANOVA followed by a Tukey’s post hoc test for multiple comparisons was used. For (A), (C), and (D), scale bars, 200 μm. For (D) and (E), N ≥ 3 organoids per group. For (E) and (F), a two-tailed t test was used to assess significance. For (B), (E), and (F), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Article Snippet: The following kits were used to quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA kit (#DY210, R&D Systems), and Mouse TNF-alpha DuoSet ELISA kit (#DY410, R&D Systems).

Techniques: Confocal Microscopy, Generated, Immunofluorescence, Expressing, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Construct

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: mCD27-based anti-murine CD70 CAR-T cells are potent against murine GB in vitro (A) Murine CD27-based construct design. (B) Transduction efficiency of primary murine T cells by flow cytometry. A non-transduced (NT) sample from each donor mouse was used to determine gating. Data gated on single live mCD3 + cells. (C) Measurement of mCD70 gene expression levels in generated OE models by RT-qPCR. N = 3 technical replicates per cell line. (D) Measurement of mCD70 on the surface of generated murine OE GB models by flow cytometry (blue histograms). Signal was compared to that of an isotype control (red histograms). (E) Quantification of secreted TNF-α in the SN of mGB/mCAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. (F) Pairwise comparisons of secreted TNF-α levels from (E) among targeting constructs. A one-way ANOVA with a post hoc Holm-Šídák test was used for significance. (G) Schematic representation of the live-cell imaging pipeline. (H) Quantification of tumor cell signal from (G) over time. A one-way ANOVA with a Dunnett’s multiple comparisons test was used with data from the t = 660 min mark. For (C) and (E), an unpaired two-tailed t test was used for significance. Data are presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant; n.d., not detected.

Article Snippet: The following kits were used to quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA kit (#DY210, R&D Systems), and Mouse TNF-alpha DuoSet ELISA kit (#DY410, R&D Systems).

Techniques: In Vitro, Construct, Transduction, Flow Cytometry, Gene Expression, Generated, Quantitative RT-PCR, Control, Enzyme-linked Immunosorbent Assay, Live Cell Imaging, Two Tailed Test